Objectives and activities

Year

Objectives

Associated activities

2007

1

Updating information and scientific data concerning the research theme. Elaboration of research methodology by brain-storming of the project team

Documentation in international data basis, access using project founds.

Assigning responsibilities of the project personnel

Elaboration in details of activity plan

2

Setting the acquisition plan and initiation of acquisition procedure of materials necessary to run the project.

Setting the acquisition plan

Initiation of acquisition procedure of materials necessary to run the project.

Objectives and activities have been achieved 100%

2008

1

Testing ethanol biosynthesis capacity of yeast and bacterial strains in specific conditions found in the phase of simultaneous hydrolysis and fermentation

Acquisition of new fermentative microbial strains
Fermentation of pretreated biomass with Saccharomyces strains

Fermentation of pretreated biomass with bacterial strains

Determination of ethanol from fermentation medium by gas-chromatography
Direct contact with international research community (abroad travels)

2

Biosynthesis of cellulase enzymes with Trichoderma and Aspergillus strains in submerse system

Cultivating Trichoderma and Aspergillus in shake flasks

Isolation and purification of cellulases from culture media

Determination of enzymatic activity of cellulase

3

Biosynthesis of cellulase enzymes with Trichoderma and Aspergillus strains in solid state fermentation system (SSF)

Cultivation of Trichoderma and Aspergillus in solid substrate

Extractionof cellulases by of solid substrate

Determination of cellulase activity

Objectives and activities have been achieved 100%

2009

1

Pretreatment of lignocellulosic biomass by acid hydrolysis and pressured steam

Pretreatment of lignocellulosic biomass with diluted sulfuric acid and autoclaving

Determination of soluble sugars and brute cellulose

Direct contact with international research community (abroad travels)

2

Pretreatment of lignocellulosic biomass by alkaline hydrolysis and pressured steam

Pretreatment of lignocellulosic biomass (straw, corn stover) with sodium hydroxide and autoclaving

Determination of soluble sugars and brute cellulose

3

Comparative determination of enzymatic activities of commercial cellulases and those produced in laboratory.

Concentration of cellulolytic enzymes by evaporation

Determination of enzymatic activity of cellulase by Peterson & Porath method

Determination of enzymatic activity of cellulase by IFPU method

Objectives and activities have been achieved 100%

2010

1

Simultaneous saccharification and fermentation of lignocellulosic biomass

Application of obtained cellulases and selected strains in Folosirea celulazelor obtinute si a tulpinilor selectionate anterior in simultaneous saccharification and fermentation of lignocellulosic biomass

Direct contact with international research community (abroad travels)

2

Integration of biomass to ethanol conversion phases for obtaining a high conversion yield

Integrated application of biomass to ethanol conversion process

Calculation of total biomass to ethanol conversion yield

Study for increasing efficiency of the process

3

Delivering of obtained results and elaboration of a research theme with international colaboration

Disemination of research results in publications of A category and ISI

Presentation of results national and international conferences

Partnership in biofuel domain with other R&D institutions

Objectives and activities have been achieved 100%